Anti-cancer Effects of the Mixture of Spirodelae Herba and Perilla Frutescens Extracts on Head and Neck Cancer Cell Line
Na Risu, 김옥수, 김병국, Yang Ying, Song Jianan, Liu Danyang, 전상미, 김옥준,
소속 상세정보
( Na Risu ) - Chonnam National University School of Dentistry Department of Oral Pathology
김옥수 ( Kim Ok-Su ) - Chonnam National University School of Dentistry Department of Periodontology
김병국 ( Kim Byung-Gook ) - Chonnam National University School of Dentistry Department of Oral Medicine
( Yang Ying ) - Chonnam National University School of Dentistry Department of Oral Pathology
( Song Jianan ) - Chonnam National University School of Dentistry Department of Oral Pathology
( Liu Danyang ) - Chonnam National University School of Dentistry Department of Oral Pathology
전상미 ( Jeon Sang-Mi ) - Chonnam National University School of Dentistry Department of Oral Pathology
김옥준 ( Kim Ok-Joon ) - Chonnam National University School of Dentistry Department of Oral Pathology
Abstract
Spirodelae Herba (SH) and Perilla Frutescens (PF) extracts have been widely used in clinical practice with various disorders for thousands of years. There are some reports regarding the anticancer effects of SH and PF each by each, but their mixture have not been investigated and their mechanisms also have not been clear. The purpose of the present study was to investigate the anticancer mechanisms and their effects of the mixture of SH and PF extracts on head and neck cancer cell line. Head and neck carcinoma KB cells were treated with SH, PF and their mixture. Anticancer effects were investigated by searching cancer cell death pathway; apoptosis and autophagy, which have been regarded to be effective and safe methods. Apoptosis, which is termed a programmed cell death, was observed by TUNNEL assay. Autophagy, which is termed a type II programmed cell death, was observed by acridine orange red staining. Additionally, the protein expressions associated with apoptosis and autophagy were detected for their mechanism by western blots. The mixture of SH and PF extracts induced autophagic and apoptotic cell death simultaneously in cancer cells. And 0.4 mg/ml of the mixture with SH and PF extracts down-regulated the expression of mTOR, however, the expressions of ATG5 and LC3-II, which induced autophagy, up-regulated. The mixture of SH and PF extracts also down-regulated the expressions of Bcl-2, but up-regulate the expressions of PARP-1 cleavage, Caspase-9 cleavage, Caspase-3 cleavage and BAX, which induced apoptosis. Taken together, these results suggested that the mixture of SH and PF extracts induce autophagic and apoptotic cell death simultaneously in head and neck cancer cells and it could be used as an alternative for anti-cancer drugs.
키워드
Spirodelae Herba (SH) and Perilla Frutescens (PF) extracts; Anti-cancer; Apoptosis; Autophagy
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